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KMID : 0923620070070040173
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Immune Network
2007 Volume.7 No. 4 p.173 ~ p.178
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Role for CD40 and CD40L Expression in Generating CD8 T Cell Response to Minor Histcompatibility Antigen, H60
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Jung Kyoung-Min
Choi Eun-Young
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Abstract
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Background: We studied the role for expression of CD40 and CD40L by CD4 and CD8 T cells in the generation of CD8 T cell response to minor histocompatibility antigen, H60. H60 is a cellular antigen to which CD8 responses require CD4 T cell help.
Methods: CD40- or CD40L-deficient mice were adoptively transferred with normal CD4 or CD8 T cells or with memory CD4 or CD8 T cells, and were immunized with male H60 congenic splenocytes to induce CD8 T cell response to H60. Peripheral blood CD8 T cell from the immunized mice were stained with the H60 tetramer.
Results: CD8 T cell response to H60 was not induced in both CD40- and CD40L-deficient mice. Adoptive transfer of CD40+/+ CD8 T cells into CD40-deficient mice did not compensate the defect in inducing CD8 T cell response to H60, while the H60-specific CD8 T cells were activated in the CD40-deficient mice that were adoptively transferred with CD40+/+ CD4 T cells. Adoptive transfer of CD40L+/+ CD4 T cells into CD40L-deficient mice induced primary CD8 T cell response for H60 and the presence of CD40L+/+ CD4 T cells was required even for memory CD8 T cells response to H60.
Conclusion: Our results suggest that the CD40-CD40L interaction mediates the delivery of CD4 T cell help to naive and memory H60-specific CD8 T cells. While the expression of CD40L by CD4 T cells is essential, signaling through CD40 on CD8 T cells is not required for the induction of CD8 T cell response to H60.
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KEYWORD
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CD8 T cell response, CD4 help, CD40, CD40L, minor histocompatibility antigen
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